ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 0.4; HindIII--3.4; BamHI--3.4; PstI--3.4; XbaI--3.4.
Contains a BstEII site 68 nt from the 5' end.
A full-length clone.
Oriented such that transcription from the SP6 promoter gives the antisense (-) strand, that can be used as a probe to detect satellite sequences.
Classification
Cucumovirus
References
Avila-Rincon MJ, et al. In vitro translation of cucumoviral satellites. I. Purification and nucleotide sequence of a cucumber mosaic virus-associated RNA 5 from cucumber mosaic virus strain S. Virology 152: 446-454, 1986.
Avila-Rincon MJ, et al. In vitro translation of cucumoviral satellites. II. CARNA 5 from cucumber mosaic virus strain S and SP6 transcripts of cloned (S)CARNA 5 cDNA produce electrophoretically comigrating protein products. Virology 152: 455-458, 1986. PubMed: 3487880
Steen MT, et al. In-vitro translation of cucumoviral satellites. III. Translational efficiencies of cucumber mosaic virus-associated RNA 5 sequence variants can be related to the predicted secondary structures of their first 55 nucleotides. Virus Genes 4: 41-52, 1990. PubMed: 1697436
